目的　观察细胞凋亡相关基因的表达。方法　天花粉蛋白诱导Ｕ９３７细胞凋亡后，用锚定引物和随机引物进行差异显示逆转录ＰＣＲ反应，回收差异条带，进行ＲＮＡ点杂交。用细胞凋亡时高表达的差异片段筛选胎肝ｃＤＮＡ文库，随后测序，行Ｎｏｒｔｈｅｒｎ杂交。结果　用细胞凋亡时高表达的差异片段筛选胎肝ｃＤＮＡ文库，获得一个１－４ｋｂ的ｃＤＮＡ克隆，该ｃＤＮＡ序列中部分碱基与人Ｂｒｕｔｏｎ○ｓ酪氨酸激酶高度同源。用１－４ｋｂｃＤＮＡ进行Ｎｏｒｔｈｅｒｎ杂交证实该基因在细胞中有３个转录本存在，其中０－７ｋｂ的ｍＲＮＡ在凋亡细胞中高表达。同时观察到Ｄｒｇ１基因高表达。结论　用差异显示逆转录ＰＣＲ方法结合ｃＤＮＡ文库筛选，寻找到１－４ｋｂ的ｃＤＮＡ片段，部分序列与Ｂｒｕｔｏｎｓ酪氨酸激酶高度同源，是与细胞凋亡有关的新基因的部分片段。凋亡相关基因Ｄｒｇ１在天花粉蛋白诱导Ｕ９３７细胞凋亡时高表达。 Objective To observe the expression of apoptosis related genes. Methods Trichosanthin induced the apoptosis of U937 cells, and the differences between anchored and random primers were analyzed by RT - PCR. The differential bands were recovered and hybridized by RNA dot blotting. Fetal liver cDNA library was screened by the differentially expressed fragment at the time of apoptosis, followed by sequencing and Northern blotting. Results Fetal liver cDNA library was screened by differentially expressed fragments at the time of apoptosis, and a 1-4kb cDNA clone was obtained. Some of the bases in this cDNA sequence were highly homologous to human Bruton’s tyrosine kinase. Northern blotting with 1-4kb cDNA confirmed that there were three transcripts in the cell, of which 0-7 kb mRNA was highly expressed in apoptotic cells. Drg 1 gene was also observed that high expression. Conclusion The cDNA fragments of 1-4 kb were screened by differential display RT-PCR and cDNA library. The partial sequences were highly homologous to Brutons tyrosine kinase and were partial fragments of new genes related to apoptosis. Apoptosis-related genes Drg 1 in Trichosanthin-induced apoptosis in U937 cells when high expression.