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目的:调查云南省大理白族自治州祥云县蚊虫中蚊媒病毒携带情况。方法:2017年8月在祥云县水涨地、清华洞捕蚊，对蚊虫分类鉴定、计数，接种至BHK-21细胞分离病毒，阳性分离物用RT-PCR扩增序列，鉴定分析病毒种类和分子特征。结果:共捕获蚊虫20 276只，其中86.87%为三带喙库蚊，7.89%为按蚊，1.29%为致倦库蚊，3.95%为其他未分类蚊种。病毒分离后获得阳性分离物17株，均为乙脑病毒I型，其中16株来自三带喙库蚊，1株来自按蚊。结论:三带喙库蚊为祥云县的主要优势蚊种和带毒蚊种；首次在祥云县分离到乙脑病毒，且均为基因I型。“,”Objective:To investigate the status of mosquito-borne viruses in mosquitoes in Xiangyun county of Dali Bai Autonomous Prefecture, Yunnan province.Methods:Mosquitoes were caught in Shuizhangdi village and Qinghua cave in Xiangyun county in August 2017. The mosquito specimens were classification, identified and counted. The specimens were innoculated in BHK-21 cells to isolate the virues. Postive cultures were used to amplify gene sequences with RT-PCR. The species and molecular characteristics of the viruses were then analyzed.Results:A total of 20 276 mosquitoes were caught. n Culex tritaeniorhynchus accounted for 86.87%, n Anopheles accounted for 7.89%, n Cx.pipiens quinquefasciatus accounted for 1.29% and others unidentified mosquitos accounted for 3.95%. Seventeen positive cultures were obtained and RT-PCR results indicated that all strains were Japanese encephalitis virus genotype I, including one stain from n Anophele and 16 from n Culex tritaeniorhynchus.n Conclusions:Culex tritaeniorhynchus is the predominant and virus-carrying mosquitoes species in Xiangyun county. This is the first time to isolate Japanese encephalitis virus in mosquitoes of Xiangyun county, and all strains were genotype I.n