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目的了解江苏口岸鼠形动物携带病原体情况,为采取相应的防治措施提供依据。方法采用夹夜法在江苏17个口岸捕获鼠形动物;通过荧光定量PCR法对捕获的鼠形动物进行鼠疫杆菌、汉坦病毒核酸检测,并对阳性标本进行测序分型。结果 418份鼠样中鼠疫杆菌检测均为阴性,汉坦病毒检测有18例阳性,阳性率为4.31%,其中15例为汉城型(SEO),其宿主均为褐家鼠,来源于连云港口岸;其余3例为汉滩型(HTN),其宿主均为黑线姬鼠,来源于南京龙潭港。结论江苏口岸鼠体中未检出鼠疫杆菌,部分口岸鼠体中检出汉滩病毒,提示这些口岸存在肾综合征出血热发生和流行的风险,应加强对相应口岸鼠形动物的监测。
Objective To understand the pathogen-carrying status of murine animals in Jiangsu Port, and to provide basis for taking corresponding control measures. Methods The murine animals were captured at 17 ports of Jiangsu Province using the method of nocturnal capture. The captured mice were tested for the presence of Yersinia pestis and Hantavirus by fluorescence quantitative PCR, and the positive specimens were sequenced. Results 418 out of the mice were negative for the detection of plague bacilli, 18 were positive for Hantavirus, and the positive rate was 4.31%, of which 15 were Seoul-type (SEO) and their hosts were Rattus norvegicus ; The remaining three cases were HANTA type (HTN), whose hosts were Apodemus agrarius, originating from Nanjing Longtan Harbor. Conclusions Hantaan virus was not detected in rat population of Jiangsu port and Hantaan virus in some port mice, suggesting the occurrence and prevalence of hemorrhagic fever with renal syndrome in these ports. Therefore, the monitoring of mouse-shaped animals should be strengthened.