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观察大黄生物活性成份大黄素对免疫功能的影响,发现大黄素可抑制不同有丝分裂原(ConA,LPS)刺激的小鼠脾细胞增殖反应,抑制ConA诱导脾细胞IL的产生。其抑制作用与大黄素浓度成正比。进一步研究发现,培养后实验组细胞存活率与对照组基本相当,在ConA刺激脾细胞增殖的晚期加入大黄素仍有明显的抑制作用,提示大黄素是通过抑制细胞DNA的合成而发挥作用,并非细胞毒的作用。
Observing the effect of emodin, a biologically active component of rhubarb, on immune function, it was found that emodin can inhibit the proliferation of splenocytes stimulated by different mitogens (ConA, LPS) and inhibit the production of IL in splenocytes induced by ConA. Its inhibitory effect is proportional to the concentration of emodin. Further study found that the survival rate of cells in the experimental group was similar to that in the control group. The addition of emodin at the late stage of ConA-stimulated splenocyte proliferation still had significant inhibitory effects, suggesting that emodin works by inhibiting DNA synthesis in cells, not The role of cytotoxicity.