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目的:探讨嗜酸性粒细胞(EOS)趋化因子局部注射后观察小鼠的肿瘤生长情况,肿瘤组织细胞因子的变化。推测EOS和肿瘤之间的相互关系。方法:用BALB/c小鼠建立黑色素瘤小鼠模型,并在种瘤部位附近局部注射eotaxin(EOS趋化因子,100μg/L),隔天注射1次。于第16天处死小鼠,取其肿瘤组织做HE染色,观察肿瘤组织局部形态结构的变化。以放射免疫法检测黑色素瘤小鼠血清以及肿瘤组织匀浆中TNF-α和IL-10的含量。结果:(1)非EOS趋化因子组和EOS趋化因子组肿瘤称重显示组间无统计学意义。(2)HE染色显示,EOS趋化因子组镜下观察到在肿瘤组织间质周围可见大量的坏死组织和炎症细胞浸润,在肿瘤组织内有较多嗜酸性粒细胞、巨噬细胞、淋巴细胞浸润。而在非EOS趋化因子组肿瘤组织内及周围EOS浸润较少,巨噬细胞也较少。(3)放射免疫法结果显示EOS趋化因子处理组肿瘤组织匀浆中TNF-α、IL-10含量明显高于非EOS趋化因子处理组肿瘤组织匀浆中TNF-α、IL-10含量。而血清中两个组的TNF-α、IL-10含量无明显差别。结论:用EOS趋化因子局部注射后,在肿瘤组织中EOS和巨噬细胞浸润明显增加,EOS对肿瘤生长无明显抑制作用。
Objective: To investigate the tumor growth and tumor cytokines in mice after local injection of eosinophils (EOS) chemokines. Speculate on the relationship between EOS and tumor. Methods: The mouse model of melanoma was established with BALB / c mice and eotaxin (EOS chemokine, 100 μg / L) was injected locally near the tumor site and injected once a day. Mice were sacrificed on the 16th day, and the tumor tissues were taken for HE staining to observe the changes of the local morphological structure of the tumor tissues. The levels of TNF-α and IL-10 in serum and tumor homogenate of melanoma mice were detected by radioimmunoassay. Results: (1) Tumor weight in non-EOS chemokine group and EOS chemokine group showed no statistical significance. (2) HE staining showed that a large number of necrotic tissue and infiltration of inflammatory cells were observed around the interstitial of the tumor tissue in the EOS chemokine group. There were more eosinophils, macrophages and lymphocytes in the tumor tissue infiltration. In the non-EOS chemokine group tumor tissue and less EOS infiltration, fewer macrophages. (3) Radioimmunoassay results showed that the content of TNF-α and IL-10 in tumor tissue homogenate of EOS chemokine group was significantly higher than that of non-EOS chemokine group . There was no significant difference in serum TNF-α and IL-10 between the two groups. CONCLUSION: EOS and macrophage infiltration in the tumor tissue after local injection with EOS chemotactic factor was significantly increased, whereas EOS had no significant inhibitory effect on tumor growth.