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聚合酶链反应(Polymerase ChainReaction,PCR)是一种在体外模拟自然DNA复制过程,用聚合酶催化物扩增核酸分子的新技术。由美国Cetus公司的Kary Mullis于1984年发明,Saiki等人于1985年首先在《Science》杂志上完整地描述了PCR技术。应用PCR技术进行体外基因扩增,可使极微量的单拷贝特定核苷酸片段在数小
Polymerase Chain Reaction (PCR) is a new technology that simulates natural DNA replication in vitro and amplifies nucleic acid molecules with polymerase enzyme. It was invented by Kary Mullis of Cetus Company in America in 1984, and Saiki et al first described the PCR technology completely in “Science” in 1985. Application of PCR technology in vitro gene amplification, can make a very small amount of single-copy specific nucleotide fragments in a small number