随着染色体脆性部位的研究不断深入,已逐步明确了一些脆性部位与某些肿瘤染色体断裂点,癌基因定位的关系。染色体脆性部位定位是进行染色体脆性部位研究的关键之一。目前采用的染色体脆性部位定位方法主要步骤为常规收获细胞、制片、直接Giemsa染色,镜下分析摄影,将染色体标本褪油褪色后再作G带,对照G带前后照片,确定脆性部位 With the deepening of the research on the fragile parts of chromosomes, the relationship between some fragile parts and some tumor chromosome breakpoints and oncogenes has been gradually clarified. The location of chromosomal fragile sites is one of the key points in the study of the chromosomal fragile sites. At present, the main steps of the localization method of chromosomal fragile parts are routine harvesting cells, direct film production, direct Giemsa staining, microscopic analysis of photography, fade of oil samples from chromosomes and then G band,