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本文证明鸡胚细胞经鸭瘟病毒感染12,24,36,40和60小时,均可观察到细胞的核仁结构。应用电子显微镜放射自显影和Bernhard染色方法,不仅从成份与功能上说明了核仁的存在,而且进一步证明在病毒DNA大量复制,核壳体大量装配与病毒成熟的细胞中,~3H尿嘧啶核苷向核仁内掺入的速率仍很快。在鸭瘟病毒感染24小时的细胞中,单位面积核仁上标记的银粒数仍相当于正常细胞的82.7％。证明在鸭瘟病毒感染的细胞中,核仁的结构不仅存在,而且持续地保持着转录RNA的功能。这与前人在其它疱疹病毒实验的有关报道完全不同,并对这种现象进行了讨论。
This paper demonstrated that chick embryo cells were infected with the duck blast virus at 12,24,36,40 and 60 hours, were observed in the cell nucleolar structure. Application of electron microscopy autoradiography and Bernhard staining, not only from the composition and function of the existence of nucleoli, and further evidence of a large number of viral DNA replication, the nuclear shell mass assembly and the virus mature cells, ~ 3H uracil nucleus The incorporation of glycosides into the nucleolus is still rapid. In the cells infected with DDP for 24 hours, the number of silver particles per unit area of nucleoli still corresponded to 82.7% of normal cells. Proved in the duck plague virus infected cells, the structure of the nucleolus not only exists, but also continue to maintain the function of transcription RNA. This is completely different from previous reports on other herpes virus experiments, and this phenomenon is discussed.