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目的优化栀子炭微波炮制工艺。方法以HPLC法测定传统炒制方法炮制的栀子炭中京尼平苷及鞣质的量作为评价指标,采用CRITIC法计算权重系数,结合Box-Behnken响应面法,优化栀子炭微波炮制工艺,并对栀子炭微波炮制品的药效进行验证。结果 CRITIC法得出炒制栀子炭京尼平苷、鞣质的权重系数分别为0.27、0.73,微波炮制栀子炭的Box-Behnken响应面法得出最佳工艺为微波功率600 W,微波时间6 min,样品质量120 g,京尼平苷、鞣质量分别为35.19、4.76 mg/g。通过药效验证表明微波炮制栀子炭与传统炒制栀子炭均能够缩短小鼠的出血时间和凝血时间,两者药效无明显区别。结论微波炮制工艺节能高效、可操作性强,该方法可以作为丰富传统炮制工艺的一种手段和方法。
Objective To optimize the processing technology of gardenia charcoal microwave. Methods HPLC method was used to determine the amount of geniposid and tannin in gardenia charcoal prepared by traditional method. The CRITIC method was used to calculate the weight coefficient and Box-Behnken response surface methodology was used to optimize the processing technology of gardenia charcoal microwave. The efficacy of Gardenia charcoal microwave products was verified. Results The CRITIC method was used to determine the weight gain of Geniposide Geniposide Geniposide with the weight coefficients of 0.27 and 0.73 respectively. The Box-Behnken Response Surface Method Time 6 min, sample quality 120 g, geniposide, tanning mass were 35.19,4.76 mg / g. Pharmacodynamic tests showed that microwave-cooked gardenia charcoal and traditional fried gardenia charcoal can shorten the bleeding time and clotting time in mice, there is no significant difference between the two. Conclusion The microwave processing technology is energy-efficient and highly operable. This method can be used as a means and method to enrich traditional processing techniques.