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蛋白质Ｃ端测序是蛋白质化学与分子生物学中一门有重要意义的技术，由Ｓｃｈｌａｃｋ和Ｋｕｍｐｆ于１９２６年提出的（异）硫氰酸法在该领域发展迅速并占主导地位．对（异）硫氰酸法的反应机理，最新进展以及标准乙内酰硫脲氨基酸制备进行了较详尽的介绍
Protein C-terminal sequencing is an important technique in protein chemistry and molecular biology. The (iso) thiocyanate method proposed by Schlack and Kumpf in 1926 has developed rapidly and dominance in this field. The reaction mechanism of (iso) thiocyanate method, the latest progress and the standard amino acid preparation of thiohydantoin were described in more detail