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目的 探讨多药耐药基因(MDR1)和多药耐药相关蛋白基因(MRP)在食管癌、贲门癌中表达的临床意义。 方法 采用逆转录-多聚酶链反应(RT-PCR),对29例食管癌、贲门癌癌组织及癌旁组织中MDR1和MRP的表达进行检测。 结果 癌组织中MDR1阳性率为65.5%,高于癌旁组织中MDR1的阳性率,为37.9%(P<0.05),癌组织MDR1信使核糖核酸(mRNA)表达水平也显著高于癌旁组织(P<0.01);癌组织的MRP阳性率为48.3%,高于癌旁组织(27.6%),但无差异(P>0.05),而癌组织MRPmRNA表达水平与癌旁组织比较则有差异(P<0.05);中、低分化肿瘤的MDR1和MRP表达阳性率增高,两基因的mRNA表达水平显著高于高分化肿瘤(P<0.05)。 结论食管、贲门癌具有内源性多药耐药性;MDR1和MRP表达与食管、贲门癌的组织学类型及TNM分期无关,但可反映其肿瘤组织分化不良的生物学特征
Objective To investigate the clinical significance of the expression of multidrug resistance gene (MDR1) and multidrug resistance protein (MRP) in esophageal and gastric cardia cancer. Methods The expression of MDR1 and MRP in 29 cases of esophageal and gastric cardia carcinoma tissues and adjacent tissues were detected by reverse transcription polymerase chain reaction (RT-PCR). Results The positive rate of MDR1 was 65.5% in cancer tissues, which was higher than that in adjacent tissues (37.9%, P <0.05). The expression of MDR1 messenger RNA (mRNA) in cancer tissues was also significantly higher than that in adjacent tissues (P <0.01). The positive rate of MRP in cancerous tissue was 48.3%, which was higher than that in paracancerous tissue (27.6%), but no difference (P> 0.05). The expression of MRP mRNA in cancerous tissue was significantly different from that in paracancerous tissue <0.05). The positive rates of MDR1 and MRP in moderate and poorly differentiated tumors were significantly higher than those in well-differentiated tumors (P <0.05). Conclusions Esophageal and gastric cardia carcinoma have endogenous multidrug resistance. The expressions of MDR1 and MRP are not related with the histological type and TNM stage of esophagus and cardia carcinoma, but they can reflect the biological characteristics of poorly differentiated tumor tissues