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目的探讨一氧化氮(NO)与心肌缺血预调置作用的相关性。方法将50只体质量300～350 g雄性健康Wistar大鼠分为对照组、缺血/再灌注损伤(I/R)组、预调置组、L-硝基精氨酸(L-NNA组)和L-精氨酸(L-Arg)+L-NNA等5组,分别观察冠脉灌注压(CPP)和心肌蛋白、乳酸脱氢酶(LDH)、环一磷酸鸟苷(cGMP)和丙二醛(MDA)含量等心肌损伤指标的变化。结果与I/R组比较,预调置组心肌蛋白漏出量明显降低、LDH漏出量显著下降、心肌MDA含量明显降低、cGMP水平则明显升高(P均<0.01),CPP降低(P<0.05);L-NNA灌流组可以阻断预调置的心肌保护作用,L-NNA+L-Arg灌流组可逆转L-NNA阻抑预调置的心肌保护作用。结论心肌缺血预调置明显提高缺血心肌对缺血缺氧的耐受力,与改善心肌I/R损伤时NO缺陷密切相关。
Objective To investigate the relationship between nitric oxide (NO) and myocardial ischemic preconditioning. Methods Fifty male Wistar rats with body weight of 300 ~ 350 g were divided into control group, ischemia / reperfusion injury (I / R) group, preconditioning group, L-NNA group (L-Arg) + L-NNA and other 5 groups were observed coronary artery perfusion pressure (CPP) and myocardial protein, lactate dehydrogenase (LDH), cyclic guanosine monophosphate (cGMP) and The changes of myocardial injury indicators such as malondialdehyde (MDA) content. Results Compared with the I / R group, the leakage of myocardial protein in the preconditioning group was significantly lower than that in the I / R group (P <0.05), the LDH leakage was significantly decreased, the myocardial MDA content was significantly decreased, while the cGMP level was significantly increased ); L-NNA perfusion group can block the preconditioning myocardial protection, L-NNA + L-Arg perfusion group can reverse the inhibition of L-NNA preconditioning of myocardial protection. Conclusions Myocardial ischemic preconditioning significantly improves the tolerance of ischemic myocardium to ischemia and hypoxia, which is closely related to the improvement of NO deficiency during myocardial I / R injury.