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通过水稻黑条矮缩病的田间鉴定发现,分蘖盛期的病状最为显著,是病症观察的最佳时期。对江苏省311份粳稻品种进行重病区田间鉴定试验,未发现对黑条矮缩病毒(RBSDV)免疫的品种。江苏省目前正在推广的24个主栽水稻品种发病率在10.0%～30.0%之间,曾推广的287份粳稻品种中,71.5%的品种发病率在10.0%～29.0%之间,其余品种发病率在29%以上。来源于日本的粳稻品种Koshihikari的黑条矮缩病发病率相对较低,籼稻高产品种桂朝2号为感病品种。利用Koshihikari/桂朝2号RILs群体进行抗RBSDV的基因定位,结果在第3染色体上标记RM7～RM5748之间检测到1个抗黑条矮缩病的QTL,来自Koshihikari的等位基因增强了水稻黑条矮缩病的抗性,携带抗性位点的家系明显提高了对RBSDV的抗性。本研究结果将为研究水稻黑条矮缩病和水稻抗黑条矮缩病分子育种提供重要信息。
Through field identification of rice black-streaked dwarf disease, it was found that the most significant symptom of tillering stage was the best period of disease observation. A field trial was conducted on 311 japonica rice varieties in Jiangsu Province. No species immunized against RBSDV was found. The prevalence of 24 main cultivated rice varieties currently being promoted in Jiangsu Province is between 10.0% and 30.0%. Among the 287 japonica rice varieties that have been popularized, the incidence of 71.5% of varieties is between 10.0% and 29.0%, and the rest of the cultivars are affected Rate above 29%. The incidence of black-streaked dwarf disease is relatively low in Koshihikari, a japonica rice derived from Japan. Guichao 2, a high-yielding indica rice, is susceptible. Using the Koshihikari / Guichao RILs population for anti-RBSDV gene mapping, one QTL for resistance to black-streaked dwarfism was detected between RM7 and RM5748 on chromosome 3, and the allele from Koshihikari enhanced rice Resistance to black-streaked dwarfism, families with resistant loci significantly increased resistance to RBSDV. The results of this study will provide important information for the study of rice black-streaked dwarf disease and molecular breeding of rice against black-streaked dwarf disease.