论文部分内容阅读
对22个省的大麦农家品种487份和3个近缘野生种的10份材料,应用SDS-PAGE、IEF及双向电泳(IEF、SDS-PAGE)进行了醇溶蛋白多肽多态性的研究,主要结果如下:(1)B醇溶蛋白多肽多态性十分显著。经SDS-PAGE可分出16种带型,归为α、β、γ、ω四组,不同的多肽带型中间也有一些多肽是共有的;(2)5种带型的地理分布表明,具有同种带型的品种分布于一定的地理区域;各区域品种所具有带型类型的数目虽然不同,但表现出一定的趋向,这种趋向似与大麦进化和生态区域有关;(3)近缘野生大麦的带型有的与农家品种带型相似,有的则相异;但一些农家品种的带型在供试近缘野生种中还未找到与之相近的带型;(4)初步讨论了醇溶蛋白多肽多态性在品种鉴定上的应用和作为栽培大麦生态区域划分辅助手段的可能性。
A total of 487 barley cultivars and 10 wild relatives from 22 provinces were studied for the polymorphisms of gliadin using SDS-PAGE, IEF and two-dimensional electrophoresis (IEF, SDS-PAGE) The main results are as follows: (1) B prolamin polypeptide polymorphism is very significant. Sixteen bands were identified by SDS-PAGE and classified into four groups of α, β, γ and ω. Some polypeptides were shared among different bands. (2) The geographical distribution of the five bands showed that there were The species of the same type distributed in a certain geographical area; although the number of the types of the types of the varieties in each area was different, they showed some tendency, which seemed to be related to the evolution and ecological area of barley. (3) Some varieties of wild barley are similar to those of peasant cultivars, while others are different. However, some peasant cultivars have not found similar patterns in their wild relatives. (4) Preliminary discussion Application of prolamin Polypeptide Polymorphism in Variety Identification and Possibility of Assorting Ecological Region for Cultivating Barley.