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为了寻找对杨树烂皮病病原菌具有高效抑制作用的木霉菌株,采用对峙培养法和生长速率法对国内外29个木霉菌株进行了筛选,并对筛选出的高效菌株的生理生态特性和分类地位进行了研究。结果表明:菌株T-33发酵液正丁醇提取物对病原菌菌丝生长的抑制率最高,达到94.2%,对孢子萌发抑制率达到100%。菌株T-33生长最适碳源为葡萄糖,最佳用量为3%(g/L);最适氮源为黄豆粉,最佳用量为0.02%(g/L);培养基最适初始p H 6;最适培养温度25℃。通过18S r DNA测序分析,ITS序列长度是583 bp,菌株T-33鉴定为Trichoderma viride。
In order to find Trichoderma strains with high inhibitory effect on the disease of poplar rot disease, 29 strains of Trichoderma were screened by confrontation culture method and growth rate method, and the physiological and ecological characteristics of the selected strains and Taxonomic status has been studied. The results showed that the n-butanol extract of strain T-33 fermentation broth had the highest inhibitory rate of 94.2% on mycelium growth of the pathogen and 100% inhibition on the spore germination. The best carbon source for strain T-33 was glucose (3% (g / L)), the optimum nitrogen source was soybean flour, and the best dosage was 0.02% (g / L) H 6; The optimum culture temperature is 25 ℃. By 18S r DNA sequencing analysis, ITS sequence length is 583 bp, strain T-33 identified as Trichoderma viride.