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目的探讨吖啶橙试验在精子DNA完整性评估中的应用价值。方法选择男性不育症患者302例。通过吖啶橙试验(AOT)与计算机辅助精液分析(CASA)检测DFI(精子DNA损伤指数,即精液标本中存在DNA损伤的精子所占的比率)和精液常规参数。结果所有302例不育患者中,DFI异常者72例,占23.84%。共260例患者精液常规参数异常,其中DFI异常50例,占19.23%;42例患者精液常规参数正常,DFI异常22例,占52.3 8%。畸形率异常组,DFI异常率为51.06%(24/47);活动率低下组,DFI异常率为38.65%(63/163);精子密度低下组,DFI异常率为36.67%(22/60)。精子活动率和精子密度与DFI均呈负相关,r分别为-0.297,-0.217,P均<0.01;精子畸形率与DFI呈显著正相关,P<0.01,r=0.352。结论评估精子DNA完整性在不育症临床上有重要作用,吖啶橙试验能够满足这一需要,值得推荐。
Objective To investigate the application value of acridine orange test in sperm DNA integrity assessment. Methods Male infertility patients were selected 302 cases. DFI (sperm DNA damage index, the ratio of sperm DNA damage in sperm samples) and sperm routine parameters were determined by acridine orange test (AOT) and computer-assisted semen analysis (CASA). Results Of all 302 infertile patients, 72 cases were abnormal DFI, accounting for 23.84%. A total of 260 patients with abnormal sperm parameters, including DFI abnormalities in 50 cases, accounting for 19.23%; 42 patients with normal parameters of semen, DFI abnormalities in 22 cases, accounting for 52.3%. The rate of abnormal DFI was 51.06% (24/47), while the rate of abnormal DFI was 38.65% (63/163). The abnormal rate of DFI was 36.67% (22/60) . Sperm motility and sperm density were negatively correlated with DFI, r were -0.297, -0.217, P <0.01; sperm deformity was positively correlated with DFI, P <0.01, r = 0.352. Conclusion The assessment of sperm DNA integrity plays a clinically important role in infertility. The acridine orange test can meet this need and is recommended.