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目的克隆人白血病细胞凋亡相关新基因TFAR15的小鼠同源序列 ,并比较其在不同种属间的序列同源性。方法利用EST(expressedsequencetag)拼排、RT PCR、DNA序列测定和计算机分析技术 ,对小鼠TFAR15进行研究。结果首次成功地进行了小鼠TFAR15全长cDNA的克隆和序列分析 ,发现小鼠TFAR15与小鼠其它cDNA没有明显的同源性 ,因此提交GenBank并被收录 ,登录号为AF159368。小鼠TFAR15和人TFAR15在核苷酸水平上有92.3 %的同源性 ,在氨基酸水平上有高达98.6 %的同源性 ;同时发现小鼠TFAR15在氨基酸水平上与线虫C14A4.11蛋白质有38 %的同源性。功能区分析发现 ,小鼠TFAR15cDNA序列含编码212个氨基酸的开放读码框架 ,有2个可能的N 糖基化位点 ,3个可能的caseinkinaseII磷酸化位点 ,4个可能的PKC磷酸化位点 ,并且可能是一种胞浆蛋白(cytoplasmicprotein)。结论小鼠TFAR15是进化上高度保守的新基因 ,可能具有重要的功能。
Objective To clone mouse homologue of TFAR15, a new gene of human leukemia, and compare its sequence homology among different species. Methods Mouse TFAR15 was studied by EST (expressed sequence tagging), RT PCR, DNA sequencing and computer analysis. Results For the first time, the full-length cDNA of TFAR15 was cloned and sequenced. It was found that there was no obvious homology between mouse TFAR15 and other cDNAs. Therefore, it was submitted to GenBank for accession number AF159368. Mouse TFAR15 and human TFAR15 have 92.3% homology at the nucleotide level and 98.6% homology at the amino acid level. At the same time, the mouse TFAR15 is found to have 38 amino acid levels with the C. elegans C14A4.11 protein % Homology. Functional domain analysis found that the mouse TFAR15 cDNA sequence contains an open reading frame encoding 212 amino acids, two possible N-glycosylation sites, three possible caseinkinaseII phosphorylation sites, four possible PKC phosphorylation sites Point, and may be a cytoplasmic protein (cytoplasmicprotein). Conclusion Mouse TFAR15 is a highly conserved new gene and may have important functions.