【摘 要】
：
Previous studies have indicated that two monoclonal antibodies (mAbs;A1-10 and H1-84) of the hemagglutinin (HA) antigen on the H1N1 influenza virus cross-react with human brain tissue.It has been proposed that there are heterophilic epitopes between the H
【机 构】
：
Shaanxi Province Research Center of Cell Immunological Engineering and Technology, Central Laborator
论文部分内容阅读
Previous studies have indicated that two monoclonal antibodies (mAbs;A1-10 and H1-84) of the hemagglutinin (HA) antigen on the H1N1 influenza virus cross-react with human brain tissue.It has been proposed that there are heterophilic epitopes between the HA protein and human brain tissue (Guo et al.in Immunobiology 220:941-946,2015).However,characterisation of the two mAbs recognising the heterophilic epitope on HA has not yet been performed.In the present study,the common antigens of influenza virus HA were confirmed using indirect enzyme-linked immunosorbent assays and analysed with DNAMAN software.The epitopes were localized to nine peptides in the influenza virus HA sequence and the distribution of the peptides in the three-dimensional structure of HA was determined using PyMOL software.Key amino acids and variable sequences of the antibodies were identified using abYsis software.The results demonstrated that there were a number of common antigens among the five influenza viruses studied that were recognised by the mAbs.One of the peptides,P2 (LVLWGIHHP191-199),bound both of the mAbs and was located in the head region of HA.The key amino acids of this epitope and the variable regions in the heavy and light chain sequences of the mAbs that recognised the epitope are described.A heterophilic epitope on H1N1 influenza virus HA was also introduced.The existence of this epitope provides a novel perspective for the occurrence of nervous system diseases that could be caused by influenza virus infection,which might aid in influenza prevention and control.
其他文献
To date,29 distinct microRNAs (miRNAs) have been reported to be expressed during herpes simplex virus infections.Sequence analysis of mature herpes simplex virus-1 (HSV-1) miRNAs revealed five sets of miRNAs that are complementary to each other:miR-H6-5p/
As the open reading frames of hepatitis B virus (HBV) genomes are overlapping,resistance mutations (MTs) in HBV polymerase may result in stop codon MTs in hepatitis B surface proteins,which are usually detected as a mixed population with wild-type (WT) HB
The study was conducted to explore the mechanisms of sex differences in the response to chronic hepatitis B (CHB) in terms of DNA methylation,SNP genotype,and gene expression.Genomic DNA was isolated from peripheral blood mononuclear cells (PBMCs) of CHB
Human immunodeficiency virus-1 capsid (HIV-1 CA) is involved in different stages of the viral replication cycle.During virion assembly,CA drives the formation of the hexameric lattice in immature viral particles,while in mature virions CA monomers assembl
Currently there is no effective antiviral therapy for SARS-CoV-2 infection,which frequently leads to fatal inflammatory responses and acute lung injury.Here,we discuss the various mechanisms of SARS-CoV-mediated inflammation.We also assume that SARS-CoV-2
The incidence of Japanese encephalitis (JE) has significantly decreased in China due to JE vaccines.In this study,we investigated the post-JE vaccination seroprevalence and protection provided by vaccinated sera against Japanese encephalitis virus (JEV) t
Echovirus 18 (E18),a serotype of Enterovirus B (EV-B) species,is an important pathogen in aseptic meningitis.El8 had rarely been detected in mainland China,but became the predominant pathogen associated with viral encephalitis (VE) and meningitis in Hebei
Hepatitis E virus (HEV) is the prototype of the family Hepeviridae and the causative agent of common acute viral hepatitis.Genetically diverse HEV-related viruses have been detected in a variety of mammals and some of them may have zoonotic potential.In t
Early etiological diagnosis is very important for the control of sudden viral infections,and requires antibodies with both high sensitivity and high specificity.Traditional antibody preparation methods have limitations,such as a long and arduous cycle,com
Severe influenza infections are often associated with the excessive induction of pro-inflammatory cytokines,which is also referred to as “cytokine storms”.Several studies have shown that cytokine storms are directly associated with influenzainduced fatal