尽管有了培养恶性疟原虫的成功经验,其它三种人体致病性疟原虫的体外培养仍很困难。用Trager与Jensen法单独培养三日疟原虫迄今未见报道。作者自一例外周血三日疟原虫感染达0.1％刚从加纳返回德国的旅游者采血,肝素抗凝,560×g离心7min,吸弃血浆和淡黄层,将红细胞以RPMI1640洗涤2次,将含6—8个核的裂殖体感染的红 Despite the successful experience of developing Plasmodium falciparum, in vitro culture of the other three human pathogenic Plasmodium species remains difficult. Plasmodium malariae alone has not been reported by Trager and Jensen methods. Authors from a sample of peripheral blood on the 3rd Malaria parasite infection up to 0.1% just returned from Ghana to Germany tourists blood, heparin anticoagulation, 560 × g centrifugal 7min, aspirated plasma and light yellow layer, the red blood cells were washed twice with RPMI1640, Will contain 6-8 nuclear schizonts infected red