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利用磷酸盐缓冲溶液中吡咯的电聚合,将葡萄糖氧化酶(GOD)包埋在聚吡咯(PPy)基质中以构成生物功能电极。讨论了溶液pH和聚合电位对酶固定化的影响,并用IR和交流阻抗谱对酶膜进行表征。GOD的固定化只有当pH>5.5时才能实现,由此推测酶是以带负电的粒子嵌入PPy的。交流阻抗谱表明这一电极具有有界多孔电极的特征。探索了酶与电子传递体Fe(CN)_6~(3-)同时固定化的可行性。电化学固定化的GOD保持其生物催化活性,酶反应表观上遵循Michealis-Menten动力学。
Utilizing the pyrrole electropolymerization in a phosphate buffer solution, glucose oxidase (GOD) is embedded in a polypyrrole (PPy) matrix to make a bio-functional electrode. The effects of solution pH and polymerization potential on enzyme immobilization were discussed. The enzyme membranes were characterized by IR and AC impedance spectroscopy. The immobilization of GOD can only be achieved when pH> 5.5, suggesting that the enzyme is embedded in PPy with negatively charged particles. The AC impedance spectrum shows that this electrode has the characteristics of a bounded porous electrode. The feasibility of simultaneous immobilization of enzyme with electron acceptor Fe (CN) 6 ~ (3-) was explored. Electrochemically immobilized GOD retains its biocatalytic activity, and the enzyme reaction appears to follow the Michealis-Menten kinetics.