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本文报道了Al-(D-色氨酸)胰岛素晶体结构的研究,其空间群为R3,晶胞参数:a_H=b_H=78.6,c_H=50.0,不对称单位包含两个分子。收集了2.2分辨率三个独立区的衍射数据并进行了处理。参考三方二锌和三方四锌猪胰岛素结构模型,以结构模型逐步逼近和立体化学制约最小二乘技术并结合差值Fourier图人工分析,对模型多次进行重建调整和精化,最终偏离因子R=0.218,独立区内两个Al色氨酸残基在电子密度图上表现十分清晰。本文对Al-(D-色氨酸)胰岛素在晶胞中的密堆积、构象之间的差异以及有关胰岛素结构与功能的一些问题进行了讨论。
In this paper, the crystal structure of Al- (D-tryptophan) insulin has been reported. Its space group is R3. The unit cell parameters are: a_H = b_H = 78.6, c_H = 50.0, and the asymmetric unit contains two molecules. Diffraction data were collected and processed in three independent regions at 2.2 resolution. With reference to the structural model of tripartite zinc and trigeminal zinc pig insulin, the model was approximated to the least square technique and the Fourier analysis of the difference Fourier diagram, and the reconstruction and adjustment of the model were repeated several times. The final deviation from the factor R = 0.218, the two Al-tryptophan residues in the independent region showed very clear on the electron density map. This paper discusses the close packing of Al- (D-tryptophan) insulin in the unit cell, the differences between conformations, and some questions about the structure and function of insulin.