论文部分内容阅读
目的分析甘肃省文县内脏利什曼病流行区人群利什曼原虫无症状感染现状,评价PCR、ELISA和rK39免疫层析试条法检测利什曼原虫无症状感染的潜能。方法2004年10月在甘肃文县对269例无内脏利什曼病现症及病史的人群采取随机取样法采集静脉血,分别用RV1-RV2和K13A-K13B两组PCR引物检测血样中的利什曼原虫特异DNA,以利什曼原虫可溶性抗原为包被抗原的ELISA法和rK39免疫层析试条法分别检测利什曼原虫特异性抗体,并比较几种检测方法的敏感性。结果PCR、ELISA和rK39免疫层析试条法检测人群利什曼原虫无症状感染的阳性率分别为30.9%(83/269)、24.2%(65/269)和0(0/269)。结论甘肃省文县内脏利什曼病流行区人群存在大量利什曼原虫无症状感染者,PCR是检测无症状感染较敏感、特异的方法。
Objective To analyze the status of asymptomatic infection of Leishmania in population of visceral leishmaniasis in Wenxian County, Gansu Province. The potential of asymptomatic infection with Leishmania was evaluated by PCR, ELISA and rK39 immunochromatography. Methods A total of 269 non-visceral leishmaniasis cases were recruited in Wenxian County, Gansu Province in October 2004. Random sampling was used to collect venous blood. Two groups of RV1-RV2 and K13A-K13B PCR primers were used to detect blood samples The specific antibodies against Leishmania were detected by ELISA using Leishmania soluble antigen as coating antigen and rK39 immunochromatographic test strip respectively. The sensitivity of several detection methods was compared. Results The positive rates of PCR, ELISA and rK39 immunochromatographic test were 30.9% (83/269), 24.2% (65/269) and 0 (0/269), respectively. Conclusion There are a large number of people with asymptomatic Leishmania infection in the population of visceral leishmaniasis in Wen County, Gansu Province. PCR is a sensitive and specific method for detecting asymptomatic infection.