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１９９４～１９９６年对直接热处理苹果试管苗脱毒法进行了研究。将继代于ＭＳ＋ＢＡ０．５～１ｍｇ１／Ｌ培养基上的苹果试管苗，置于光照培养箱内，每天照光１４ｈ，温度控制在３７℃±１℃，热处理２８天后，剥取０．５ｍｍ左右的茎尖进行培养，获得的试管苗用ＰＡＳ－ＥＬＩＳＡ（Ａ蛋白双夹心—酶联免疫吸附）法和指示植物法进行检测，其中有８个苹果品种脱去了苹果褪绿叶斑病毒（ＡＣＬＳＶ）和苹果茎沟病毒（ＡＳＧＶ）。对ＡＳＧＶ和ＡＣＬＳＶ的脱除率分别为８１．８％和７８．６％，说明直接对苹果试管苗进行热处理，再剥取茎尖进行培养是脱除苹果病毒，特别是ＡＳＧＶ行之有效的方法。
1994-1996 Direct heat treatment of apple tube seedling detoxification were studied. Will be on behalf of the MS + BA0.5 ~ 1mg1 / L medium of apple plantlets, placed in a light incubator, light every day 14h, the temperature was controlled at 37 ℃ ± 1 ℃, heat treatment for 28 days, stripping about 0.5mm The tips of the shoots were cultured, and the test plantlets obtained were tested by PAS-ELISA (A double-sandwich enzyme-linked immunosorbent assay) and the plant index method. Among them, 8 apple cultivars were removed ACLSV Apple stem groove virus (ASGV). The removal rates of ASGV and ACLSV were 81.8% and 78.6%, respectively. This indicated that it is an effective method to remove apple virus, especially ASGV, by directly heat-treating apple plantlets and then stripping the tips of the shoots .