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用花粉管通道法将新疆大赖草DNA导入普通春小麦花培品系761（主穗长9．5cm），在导入后代D3代中筛选出一个大穗、晚熟变异株，经常规选有获得大穗（平均主穗长15.2cm，50个系）、多粒的转化后代。以地高辛标记的4个大麦高度重复序列克隆为探针，对供体、受体和转化后代进行Southern杂交分析。结果表明转化后代中出现了受体没有而供体具有的特异带，证明新疆大赖革DNA片段已整合到受体基因组中。因此认为外源DNA直接导入技术是克服小麦与其近缘野生种间杂交不亲和性的一条可行途径。
The pollen tube pathway was used to introduce the DNA from Larvae of Xinjiang to the anther culture cultivar 761 (the main spike length 9.5cm) of the spring wheat, and a large panicle and late maturing mutant were selected from the D3 generation of the introduced progenies. (Average main ear length 15.2cm, 50 lines), multi-grain transformed progeny. Four barley highly repeatable clones labeled with digoxigenin were used as probes for Southern hybridization analysis of donor, recipient and transformed progeny. The results showed that there was no specific receptor in the transformed progeny but the donor showed that the DNA fragment of Daligee in Xinjiang had been integrated into the genome of the recipient. Therefore, it is considered that the direct introduction of foreign DNA is a feasible way to overcome the incompatibility of wheat and its wild relatives.