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目的 探讨膈肌纤维肌质网是否存在非序列依赖性DNA结合蛋白 ,并观察它与膈肌功能的关系。方法 Wistar大鼠随机分为对照组、急性气胸组和心痛定组 ,差速离心法分离大鼠膈肌纤维肌质网 (sarcoplasmicreticulum ,SR)膜蛋白 ,采用Western免疫印迹技术分离出SR非序列依赖性DNA结合蛋白 ,并观察此蛋白在急性气胸和给予心痛定时的变化特征。结果 大鼠膈肌SR上存在非序列依赖性DNA结合蛋白 ,相对分子质量分别为 6 0 0 0 0道尔顿和 780 0 0道尔顿。急性气胸时和给予心痛定时 ,非序列依赖性DNA结合蛋白的量有变化。结论 大鼠膈肌纤维SR上存在非序列依赖性DNA结合蛋白 ,该DNA结合蛋白可能与膈肌功能状态和Ca2 + 信号系统密切相关 ,它在膈肌和骨骼肌的病理生理过程中起重要作用。
Objective To investigate whether there is a non-sequence-dependent DNA-binding protein in the sarcoplasmic reticulum of diaphragmatic muscle fibers and to observe its relationship with diaphragmatic muscle function. Methods Wistar rats were randomly divided into control group, acute pneumothorax group and xintongding group. The sarcoplasmic reticulum (SR) membrane protein of rat diaphragm was isolated by differential centrifugation. The non-sequence dependent SR was isolated by Western blotting DNA binding protein, and observe the characteristics of this protein in the acute pneumothorax and give heartache timing. Results There was a non-sequence-dependent DNA-binding protein in the SR of rat diaphragm. The relative molecular masses were 60,000 Daltons and 780,000 Daltons, respectively. Acute pneumothorax and given heartache timing, non-sequence-dependent changes in the amount of DNA-binding protein. Conclusion There is a non-sequence-dependent DNA-binding protein in SR of diaphragmatic muscle fibers. This DNA binding protein may be closely related to diaphragmatic functional status and Ca2 + signaling system. It plays an important role in the pathophysiology of diaphragmatic muscle and skeletal muscle.