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Na+/H+反向转运蛋白可调控细胞质pH值、钠离子浓度和细胞体积,从而减轻盐胁迫对植物的伤害。利用发根农杆菌(Agrobacterium rhizogenes)介导法,向大豆根系导入由CaMV35S启动子调控的Na+/H+反向转运蛋白编码基因GmNHX1的cDNA序列,通过该基因的过量表达,提高大豆的耐盐性。通过潮霉素筛选、GUS染色及RT-PCR检测,确认获得了转GmNHX1基因的大豆发状根。对转基因发状根耐盐性分析表明:在100、150和200 mmol.L-1的NaCl胁迫下,置于MS固体培养基中的转基因离体发状根的长度和重量增加值均显著大于对照。带有转基因发状根的子叶及复合体植株在盐胁迫条件下也具有较强的生存能力。试验证明,过表达GmNHX1基因能够显著提高转基因发状根的耐盐性,该结果为利用GmNHX1基因进行大豆耐盐性的改良提供了依据。
Na + / H + antiporter can regulate the cytoplasm pH value, sodium ion concentration and cell volume, thereby reducing salt stress on plant damage. Using Agrobacterium rhizogenes-mediated method, the cDNA sequence of GmNHX1, a gene encoding Na + / H + antiporter protein regulated by CaMV35S promoter, was introduced into the soybean root and the salt tolerance of soybean was increased by overexpression . Through the hygromycin screening, GUS staining and RT-PCR detection, we confirmed that the transgenic hairy root of GmNHX1 was obtained. The analysis of salt-tolerance of transgenic hairy roots showed that the length and weight gain of transgenic hairy roots placed in MS solid medium were significantly higher than those under 100, 150 and 200 mmol.L-1 NaCl stress Control. Cotyledons and composite plants with transgenic hairy roots also have strong viability under salt stress conditions. The results showed that overexpression of GmNHX1 gene could significantly increase the salt tolerance of transgenic hairy roots, which provided a basis for the improvement of soybean salt tolerance by GmNHX1 gene.