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本文旨在了解抗孕-53及其类似物SIPPR-113对着床期及阉割后小鼠子宫内膜形态的影响。选用ICR成年雌小鼠,实验分两部分进行。一、交配后小鼠。按抗着床最小有效剂量处理动物。二、切除卵巢后小鼠。按上述剂量的1/4～1/2处理动物。并与雌二醇及黄体酮进行对比。全部小鼠子宫用石蜡及Epon 812包埋,制备组织学切片、半薄切片(1μm)及超薄切片,光镜和电镜下观察。得到一致的结果:抗孕-53及SIPPR-113能促进小鼠子宫内膜发育及腺体生长,细胞核有丝分裂增多,并引起子宫及基质水肿。在第一部分实验中能抑制一部分着床期腺体的分泌。电镜图像中显示内膜细胞中溶酶体较多,并可见脂滴,少数基质细胞中微丝较多。SIPPR-113显示出与抗孕-53相似的作用,此二化合物具有雌激素样作用。
This article aims to understand the impact of anti-pregnancy -53 and its analog SIPPR-113 on implantation and post-castration mouse endometrial morphology. ICR adult female mice were selected, and the experiment was divided into two parts. First, after mating mice. The animals were treated with the least effective dose of anti-implantation. Second, after ovariectomized mice. Animals were treated 1/4 to 1/2 of the above dose. And compared with estradiol and progesterone. All mice uterus were embedded with paraffin and Epon 812, histological sections, semi-thin section (1μm) and ultra-thin sections were prepared and observed under light microscope and electron microscope. Consistent results: Anti-pregnancy -53 and SIPPR-113 can promote mouse endometrial development and gland growth, increased mitotic cell nucleus, and cause uterine and stromal edema. In the first part of the experiment can inhibit part of the implantation of glandular secretion. Electron microscopy showed more lysosomes in endometrial cells with lipid droplets visible and more microfilaments in a few stromal cells. SIPPR-113 showed a similar effect to that of anti-pregnancy-53, both of which have estrogen-like effects.