This study focuses on the evaluation of the differences of physical fitness among soccer players, handball players, and basketball players, and the investigation of the physical fitness structures of those players. The participants consisted of 160 elite players selected from university teams. Fourteen tests related to health and motor fitness were conducted. The results were subjected to a multivariate analysis of variance (MANOVA) to test the mean vector differences among the three groups of different sport. Nine out of the fourteen tests were of significantly discriminating results regarding to different group, which include side step, abdominal strength, shuttle running, 100m running, pull-up, 1,500m running, trunk flexion, grip strength and broad jump, as determined by a stepwise regression approach. MANOVA showed that there was a significant difference (p< 0.001) of the mean vectors of the 9 tests among the three events. Discriminant function analysis showed that three discriminant functions were s This study focuses on the evaluation of the differences of physical fitness among soccer players, handball players, and basketball players, and the investigation of the physical fitness structures of those players. to health and motor fitness were conducted. The results were subjected to a multivariate analysis of variance (MANOVA) to test the mean vector differences among the three groups of different sport. Nine out of the fourteen tests were of significantly discriminating results versus to different group , which include side step, abdominal strength, shuttle running, 100m running, pull-up, 1,500m running, trunk flexion, grip strength and broad jump, as determined by a stepwise regression approach. MANOVA showed that there was a significant difference (p <0.001) of the mean vectors of the 9 tests among the three events. Discriminant function analysis showed that three discrimina nt functions were s