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本文研究低密度脂蛋白（ＬＤＬ）对内皮细胞表达促炎性细胞因子白细胞介素６（ＩＬ－６）和肿瘤坏死因子（ＴＮＦ）的影响及１７β－雌二醇的保护作用。以体外培养的人脐静脉内皮细胞（ＨＵＶＥＣ）为模型。将ＬＤＬ与ＨＵＶＥＣ孵育及ＨＵＶＥＣ经它莫西芬（Ｔａｍｏｘｉｆｅｎ）和／或１７β－雌二醇预处理后，再与ＬＤＬ孵育，检测培养液中ＩＬ－６和ＴＮＦ活性。发现ＬＤＬ可明显增加内皮细胞表达ＩＬ－６和ＴＮＦ。ＬＤＬ１００μｇ／ｍｌ时，ＴＮＦ为０．７４±０．０９（ｎｇ／ｍｌ），ＩＬ－６为９１６４±７３９（ｕ／ｍｌ），与对照组相比Ｐ＜００１；经１７β雌二醇预处理后，细胞在ＬＤＬ作用下，ＩＬ－６和ＴＮＦ活性与对照组相比无显著差异（Ｐ＞００５）；它莫西芬和１７β－雌二醇处理后，再加ＬＤＬ，ＩＬ－６和ＴＮＦ活性增高，与对照组相比有显著差异。实验结果表明：ＬＤＬ可促进血管内皮细胞高表达促炎性细胞因子ＩＬ－６和ＴＮＦ。雌激素可明显抑制ＬＤＬ引起的内皮细胞高表达ＩＬ－６和ＴＮＦ，从而产生对内皮细胞的保护作用。此作用可被它莫西芬拮抗。因此雌激素保护内皮细胞的作用，可能部分通过内皮细胞上雌激素受体。
This article studies the effects of low density lipoprotein (LDL) on the expression of pro-inflammatory cytokines interleukin-6 (IL-6) and tumor necrosis factor (TNF) in endothelial cells and the protective effect of 17β-estradiol. Cultured human umbilical vein endothelial cells (HUVEC) as a model. LDL was incubated with HUVEC and HUVECs were pretreated with Tamoxifen and / or 17β-estradiol, and then incubated with LDL to detect IL-6 and TNF activity in the culture medium. LDL was found to significantly increase endothelial cells expressing IL-6 and TNF. LDL at 100μg / ml, TNF 0.74 ± 0.09 (ng / ml), IL-6 9164 ± 739 (u / ml), compared with the control group P <001; 17β After pretreatment with estradiol, the activities of IL-6 and TNF in cells under the action of LDL had no significant difference compared with the control group (P> 005); after treated with tamoxifen and 17β-estradiol, Plus LDL, IL-6 and TNF activity increased compared with the control group were significantly different. The experimental results show that LDL can promote the high expression of proinflammatory cytokines IL-6 and TNF in vascular endothelial cells. Estrogen can obviously inhibit the high expression of IL-6 and TNF in endothelial cells induced by LDL, and thus produce the protective effect on endothelial cells. This effect can be antagonized by tamoxifen. Therefore, the role of estrogen in the protection of endothelial cells may partially through the estrogen receptor on endothelial cells.