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目的研究甘肃不同产区栽培当归的遗传多样性。方法应用ISSR分子标记技术对甘肃省41个居群的栽培当归样本进行分析,利用Popgene 32软件分析Nei’s基因多样性指数(H)等遗传信息参数,应用Ntsys软件构建亲缘关系UPGMA聚类图。结果 8条引物共检测到154个位点,其中多态性位点119个,多态位点百分率(PPB)为77.27%。栽培当归居群间的H为0.222 9,Shannon’s多样性信息指数(I)为0.337 4,种群间基因分化系数(Gst)为0.683 9,基因流(Nm)为0.2311,遗传距离变化范围0.042 9~0.327 8。结论甘肃栽培当归遗传多样性在物种水平上较高;居群间遗传多样性水平明显高于居群内;居群间遗传分化程度大,且基本无基因交流。
Objective To study the genetic diversity of Angelica sinensis cultivated in different areas of Gansu Province. Methods ISSR molecular markers were used to analyze cultivars of Angelica sinensis from 41 populations in Gansu Province. Populus 32 software was used to analyze genetic information such as Nei’s gene diversity index (H), and phylogenetic UPGMA clustering was constructed using Ntsys software. Results A total of 154 loci were detected by 8 primers, of which 119 were polymorphic and the percentage of polymorphic loci (PPB) was 77.27%. H was 0.222 9, Shannon’s diversity index (I) was 0.337 4, the coefficient of genetic differentiation (Gst) was 0.683 9, the gene flow (Nm) was 0.2311, the genetic distance range was 0.042 9 ~ 0.327 8. Conclusion The genetic diversity of Angelica sinensis cultivated in Gansu is higher at species level. The genetic diversity among populations is obviously higher than that in populations. The genetic differentiation among populations is large with no gene exchange.