论文部分内容阅读
为了解多粘菌素Ｂ（ＰＢ）对内耳碳酸酐酶（ＣＡＨ）的影响，用Ｈａｎｓｓｏｎ法和Ｙｏｋｏｔａ法观察豚鼠鼓室注射生理盐水或ＰＢ１万Ｕ／ｍｌ后内耳ＣＡＨ的定位。结果发现生理盐水组豚鼠内耳ＣＡＨ分布广泛，血管纹、内外毛细胞表现为极强阳性，前庭暗细胞表现为强阳性。ＰＢ组内耳ＣＡＨ活性普遍减低，电镜下外毛细胞内ＣＡＨ反应产物几乎消失，并且出现细胞水肿。推测ＰＢ破坏细胞膜的通透性后影响ＣＡＨ的活性，加重对内耳的损害
In order to understand the effect of polymyxin B (PB) on carbonic anhydrase (CAH) in the inner ear, the localization of CAH in the inner ear of Guinea pigs was observed by tympanic injection of normal saline or PB 10,000 U / ml with Hansson method and Yokota method. The results showed that the CAH of the inner ear of guinea pigs in the saline group were widely distributed. The vascular lines, the inner and outer hair cells showed extremely strong positive, and the vestibular dark cells showed strong positive. The CAH activity in the inner ear of PB group was generally reduced, and the product of CAH reaction in outer hair cells almost disappeared under electron microscope, and cell edema appeared. Speculated that PB destruction of cell membrane permeability after affecting the activity of CAH, aggravating the damage to the inner ear