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目的：检测近交２系豚鼠肝癌细胞ｌｉｎｅ１０分泌的生长因子ＴｕＧＦ（ｓ）对人肝上皮癌细胞（ＢＥＬ－７４０２）生长的影响。方法：集落形成测定法检测ＴｕＧＦ（ｓ）对ＢＥＬ－７４０２细胞在软琼脂培养基内生长的影响。ＭＴＴ法测定ＴｕＧＦ（ｓ）对ＢＥＬ－７４０２细胞在液体培养基内增殖的影响。结果：软琼脂培养基内培养时ＴｕＧＦ（ｓ）对ＢＥＬ－７４０２细胞无集落形成促进或抑制作用；单层培养的条件下，ＴｕＧＦ（ｓ）对ＢＥＬ－７４０２细胞在１×１０５个／ｍｌ接种细胞浓度下，具有促进细胞生长的作用；而在１×１０４个／ｍｌ细胞浓度下，则为抑制细胞生长的作用。结论：ＴｕＧＦ（ｓ）对ＢＥＬ－７４０２细胞体外增殖的影响依实验条件的不同具有复杂的生物学调节作用
OBJECTIVE: To investigate the effect of growth factor TuGF (s) secreted by line10 of inbred mouse hepatocellular carcinoma cell line 2 on the growth of human hepatic epithelial cancer cells (BEL-7402). METHODS: Colony formation assay was used to determine the effect of TuGF(s) on the growth of BEL-7402 cells in soft agar medium. The effect of TuGF(s) on proliferation of BEL-7402 cells in liquid medium was measured by MTT assay. Results: TuGF(s) did not promote colony formation or inhibition of BEL-7402 cells when cultured in soft agar medium; TuGF(s) inoculated BEL-7402 cells at 1×10 5 cells/ml under monolayer culture conditions. At the cell concentration, it has an effect of promoting cell growth; at a cell concentration of 1×10 4 cells/ml, it is an effect of inhibiting cell growth. Conclusion: The effect of TuGF(s) on the proliferation of BEL-7402 cells in vitro has a complex biological regulation depending on the experimental conditions.