以MTT法观察补肾和柔肝中药含药血清对体外软骨细胞的影响。结果 :在两次连续灌胃 3h后所取血清浓度为 5 %和 10 %时 ,对软骨细胞有促进增殖的作用 ;血清浓度为 5 %时 ,两药之间作用无差异 ,而 10 %时 ,补肾方作用优于柔肝方。为便于以下比较 ,将血清浓度定在 5 % ;长时间 ( 8d)灌胃和短时间 ( 1d)所取血清对软骨细胞的增殖作用无差异 ;以 0 5、1、5倍等效剂量灌胃所取血清对软骨细胞的增殖作用未呈量效依赖关系 ,其中以等效剂量最好 MTT assay was used to observe the effect of Bushen and Rougan medicine-containing serum on chondrocytes in vitro. RESULTS: When serum concentrations of 5% and 10% were taken after two consecutive intragastric administrations for 3 hours, the proliferation of chondrocytes was promoted; when the serum concentration was 5%, there was no difference between the two drugs, and 10%. , Kidney side effect is better than Rougan side. In order to facilitate the following comparisons, the serum concentration was set at 5%; for a long time (8d) gavage and a short time (1d), there was no difference in the proliferation of chondrocytes; 0.55, 1 and 5 times the equivalent dose The serum from the stomach had no dose-effect dependence on the proliferation of chondrocytes, and the equivalent dose was the best.