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目的：探讨ＩｇＨ胚系Ｃμ基因表达在白血病发病中的意义。方法：应用逆转录聚合酶链反应对各型急、慢性白血病５９例６３份骨髓标本进行免疫球蛋白重链（ＩｇＨ）胚系Ｃμ基因转录本检测。结果：在多种急、慢性白血病亚型中均可以检出胚系Ｃμ链基因的转录。在急性髓系白血病（ＡＭＬ）和急性淋巴细胞白血病（ＡＬＬ）中分别为６５．４％和７５．０％；而慢性粒细胞白血病急变期４例和慢性淋巴细胞白血病２例均呈阳性。ＩｇＨ胚系基因Ｃμ转录本阳性患者中ＨＬＡＤＲ阳性表达者占８０．０％；而在ＡＭＬ伴Ｃμ（＋）者，ＨＬＡＤＲ阳性表达者占９２．９％。对照组均为阴性。结论：相当多的髓系白血病，其白血病细胞系似源于髓系／淋巴系共同干细胞水平，或伴有不可逆性基因重排程序错误及淋巴系、髓系系列间不稳定性
Objective: To investigate the significance of Cμ gene expression in IgH germline in the pathogenesis of leukemia. METHODS: Reverse transcription-polymerase chain reaction (RT-PCR) was used to detect transcripts of Cμ gene of immunoglobulin heavy chain (IgH) germline in 63 bone marrow specimens from 59 cases of acute and chronic leukemia. RESULTS: The transcription of the Cμ chain gene was detected in many acute and chronic leukemic subtypes. In acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL), they were 65.4% and 75.0%, respectively; while in the acute phase of chronic myeloid leukemia, 4 cases and 2 cases of chronic lymphocytic leukemia were positive. IgH germline gene Cμ transcript positive patients accounted for 80.0% of HLA DR positive expression; while in AML with Cμ (+), HLA DR positive expression accounted for 92.9%. The control group was negative. CONCLUSIONS: In myeloid leukemia, the majority of myeloid leukemia cell lines appear to originate from myeloid/lymphoid stem cell levels, or with irreversible gene rearrangement errors and lymphatic and myeloid series instability.