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目的比较咔哒唑胺与利奈唑胺对不同基因和毒素型别的艰难梭菌的抑菌效果,以及咔哒唑胺对艰难梭菌芽孢形成的影响。方法采用咔哒唑胺与利奈唑胺对10株不同基因型别的艰难梭菌、1株产气荚膜梭菌和1株脆弱拟杆菌进行药物敏感性实验,获得各菌株测试药物对应的最低抑菌浓度(MIC)值。选取其中1株艰难梭菌ATCC BAA-1803,核糖体027型高毒株,试验咔哒唑胺对菌株芽孢形成的影响,从而对药物进行评价。结果艰难梭菌咔哒唑胺MIC为0.25μg/ml(0.03~0.25μg/ml),利奈唑胺MIC为4.0μg/ml(0.5~16.0μg/ml),艰难梭菌咔哒唑胺MIC值低于利奈唑胺16倍。2种抗生素对不同基因型别与毒素型别的艰难梭菌均具有良好抑菌效果,同时对产气荚膜梭菌也具有抑菌效果。咔哒唑胺对脆弱拟杆菌不产生抑菌效果,利奈唑胺对其有抑菌效果。咔哒唑胺能抑制艰难梭菌芽孢形成。结论临床常见的不同基因型和毒素型别的艰难梭菌对咔哒唑胺敏感,咔哒唑胺可用于治疗艰难梭菌感染。
OBJECTIVE To compare the bacteriostasis of carbachol with linezolid against C. difficile of different gene and toxin types, and the effect of carbazocine on C. difficile sporulation. Methods The data of 10 strains of Clostridium difficile, 1 Clostridium perfringens and 1 Bacteroides fragilis were tested using carbazoxamine and linezolid. The drug sensitivity of each strain was the lowest Bacterial concentration (MIC) value. One of the strains, C. difficile ATCC BAA-1803 and ribotype 027, was selected to test the effect of carbachol on the spore formation of the strain, and the drug was evaluated. Results MIC of C. difficile carbachodamine was 0.25μg / ml (0.03 ~ 0.25μg / ml), Linezolid MIC was 4.0μg / ml (0.5 ~ 16.0μg / ml) Linezolid 16 times. The two antibiotics have good antibacterial activity against Clostridium difficile of different genotypes and toxin types, and also have antibacterial activity against Clostridium perfringens. Carbazolidine Bacteroides fragilis does not produce antibacterial effect, linezolid antibacterial effect. Carbazolam inhibits C. difficile sporulation. Conclusions Clostridium difficile is susceptible to different genotypes and toxins commonly encountered in the clinic, and carbazole can be used to treat C. difficile infection.