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从11年生日本落叶松(Larix leptorepis)带顶芽枝条切取4毫米长的茎端部分,经67天培养后,发育成25毫米长的叶条(Shoot);从2年生苗带顶芽枝条切取5毫米长的茎端,经培养发育成为30毫米长的叶条。叶条和反复切分的茎段,都能形成腋茅。培养196天后,一个叶条共形成9个腋芽,总生长量约75毫米。从25年生优树无性系枝端冬芽切取的茎端,经107天培养后,开叶并长出4条红色的根,其中生长最好的一条根,直径为1.5毫米,长20毫米。但这种发育良好的叶条和生了根并带有针叶的芽,为数不多。
From the 11-year-old Larix leptorepis shoots with apical shoots, a 4 mm long stem-end fraction was cut and grown to a length of 25 mm Shoot after 67 days of culture; Stems 5 mm long, grown to 30 mm long leaves. Leaves and repeatedly sliced stems, can form the axillary. After culturing for 196 days, a total of 9 axillary buds were formed in one leaf blade with a total growth of about 75 mm. The stems cut from the 25-year-old tree clones of the late-stem winter buds, after 107 days of culture, opened and grew 4 red roots, of which the best growing ones were 1.5 mm in diameter and 20 mm in length. However, the well-developed leaves and sprouted buds are few.