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目的比较研究乙型肝炎病毒(HBV)转基因小鼠和C57BL/6小鼠骨髓间充质干细胞(bone marrowmesenchymal stem cells,BMSCs)的分离培养体系。方法采用贴壁培养法建立HBV转基因小鼠和C57BL/6小鼠BMSCs分离培养体系,通过在培养液中添加不同浓度的血清进行培养,分析HBV转基因小鼠和C57BL/6小鼠BMSCs细胞形态和数量的差异。结果 C57BL/6小鼠原代BMSCs生长迅速,按1∶3传代后呈漩涡状生长,形态类似成纤维细胞。HBV转基因小鼠原代BMSCs生长缓慢;以1∶2传代后生长缓慢,有明显的血清依赖性及密度依赖性。结论在相同的生长环境下,不同血清浓度的培养液中,HBV转基因小鼠BMSCs生长较正常C57BL/6小鼠的BMSCs生长明显缓慢。
Objective To compare the isolation and culture of BMSCs from hepatitis B virus (HBV) transgenic mice and C57BL / 6 mice. Methods The isolation and culture system of BMSCs from HBV transgenic mice and C57BL / 6 mice was established by adherence culture method. The culture of BMSCs in HBV transgenic mice and C57BL / 6 mice was analyzed by adding different concentrations of serum in culture medium. The difference in quantity. Results The primary BMSCs of C57BL / 6 mice grew rapidly. After passage 1: 3, the primary BMSCs grew swirling like fibroblasts. The primary transgenic BMSCs of HBV transgenic mice grew slowly. After 1: 2 passage, the primary BMSCs grew slowly and had obvious serum-dependent and density-dependent. Conclusion In the same growth environment, the BMSCs of HBV transgenic mice grew more slowly than those of normal C57BL / 6 mice in different serum concentrations.