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目的;观察维甲酸(RA)对实验性大肠癌的逆转治疗作用。方法:应用维甲酸对大鼠大肠癌的诱发过程进行干预治疗,观察癌变率及增殖细胞核抗原(PCNA)和核仁组成区嗜银蛋白(AgNOR)的表达。结果:维甲酸治疗组(Ⅱ组)大肠癌的发生率显著低于未加维甲酸治疗的对照组(Ⅰ组)。在诱癌的中晚期PCNA指数及AgNOR数亦显著低于Ⅰ组(P<0.01)。Ⅰ、Ⅱ组的PCNA指数及AgNOR数显著高于未用诱癌剂的Ⅲ、Ⅳ组(P<0.01)。组内对比结果显示,Ⅰ组PCNA指数及AgNOR数有随着诱癌时间延长而增加的趋势,差异有显著性(P<0.05),而Ⅱ、Ⅲ、Ⅳ组内比较差异无显著性(P>0.05)。结论:维甲酸可完全或部分阻逆实验性大肠癌的癌变过程,降低其发生率,为临床应用维甲酸防治大肠癌提供了理论资料。
Objective To observe the effect of retinoic acid (RA) on reversal of experimental colorectal cancer. Methods: Retinoic acid was used to induce the induction of colorectal cancer in rats. The rates of carcinogenesis and expression of proliferating cell nuclear antigen (PCNA) and nucleolar organizer region AgNOR were observed. Results: The incidence of colorectal cancer in the retinoic acid treatment group (group Ⅱ) was significantly lower than that in the control group without retinoid acid treatment (group Ⅰ). The PCNA index and AgNOR number of middle and late stages of cancer induction were also significantly lower than those in group Ⅰ (P <0.01). The PCNA index and AgNOR number in group Ⅰ and Ⅱ were significantly higher than those in group Ⅲ and Ⅳ without inducing carcinogens (P <0.01). The results of in-group comparison showed that the PCNA index and AgNOR number in group Ⅰ tended to increase with the duration of cancer induction (P <0.05), while there was no significant difference in group Ⅱ, Ⅲ and Ⅳ (P > 0.05). Conclusion: Retinoic acid can completely or partially hinder the carcinogenesis of experimental colorectal cancer and reduce its incidence, providing theoretical data for the clinical application of retinoic acid in the prevention and treatment of colorectal cancer.