目的建立大鼠血浆中栀蒡热毒平方有效组分中栀子苷、牛蒡子苷和黄芩苷的HPLC血药浓度测定方法。方法血浆样品采用甲醇-乙腈(1∶1)沉淀蛋白,HPLC测定血药浓度。色谱柱为Diamonsil C18柱(4.6 mm×150 mm,5μm),流动相乙腈-0.2%磷酸水梯度洗脱,流速1.0 mL/min,检测波长238 nm和278 nm。结果各成分的最低检测浓度(S/N>3)在0.23~0.32 mg/L,平均回收率均在85%以上,日内、日间精密度及稳定性的RSD均小于10%。结论该方法简便、准确,可作为栀蒡热毒平方血药浓度定量分析方法 。 Objective To establish HPLC method for the determination of geniposide, arctiin and baicalin in the effective components of Radix Scutellariae in rats plasma. Methods The plasma samples were precipitated with methanol-acetonitrile (1: 1) and the plasma concentrations were determined by HPLC. The chromatographic column was Diamonsil C18 column (4.6 mm × 150 mm, 5 μm). The mobile phase was eluted with a gradient of acetonitrile-0.2% phosphoric acid. The flow rate was 1.0 mL / min and the detection wavelength was at 238 nm and 278 nm. Results The minimum detectable concentration (S / N> 3) of each component ranged from 0.23 to 0.32 mg / L and the average recoveries were over 85%. The RSDs of intra- and inter-day precision and stability were less than 10%. Conclusion The method is simple and accurate and can be used as a quantitative analysis method for the blood concentration of daidzein and venom.