以吉林省扶余县三井子镇沙壤土为供试土壤,通过对花生根瘤菌的采集、菌株的分离、纯化和原寄主品种的回接等盆栽试验,筛选出适合吉林省不同花生品种的优良菌株(2644、2656、2697)及其组合(2644+2656、2644+2697),并对其进行生理生化特性鉴定。结果表明,2644为快生型根瘤菌,表现出相对较强的固氮酶活性,2656、2697为慢生型根瘤菌,也具有较强的固氮活性。修正了花生根瘤菌无快生型或快生型固氮活性低的传统说法。同时这3种优良菌株中两种或两种以上组合接种效果更佳,是生产上推广应用的优选菌株,这为引进优良菌种和组合接菌提供了理论依据。 Taking sandy loam soil in Sanjingzi Town, Fuyu County, Jilin Province as test soil, the best strain (strain) suitable for different peanut varieties in Jilin Province was screened out through pot experiments such as collection of rhizobium, separation and purification of strains and back connection of original host species 2644,2656,2697) and their combination (2644 + 2656,2644 + 2697), and its physiological and biochemical characteristics of identification. The results showed that 2644 was a rapid-growth rhizobia, which showed relatively strong nitrogenase activity. 2656,2697 was slow-growth rhizobia and also had strong nitrogen fixation activity. Fixed a non-fast-growing peanut rhizobia or fast-acting nitrogen fixation activity of the traditional statement. At the same time, two or more of the three excellent strains were better inoculation, which was the preferred strain for the promotion of production. This provided the theoretical basis for the introduction of excellent strains and combined inoculation.