论文部分内容阅读
目的:利用RNA干扰(RNAinterfering,RNAi)技术构建HnRNPA2/B1重组质粒,并行序列分析。方法:分别将21bP长短的HnRNPA2/B1靶序列,中间为7bP序列间隔的反向重复序列,置入PsiRNA-hH1neoG2质粒中,产生重组质粒PSiRNA-hH1neoG2HnRNPA2/B1。结果:将重组质粒作测序分析,经测序鉴定确定为所需序列。结论:特异性短发夹环RNA(ShRNA)真核表达载体PSiRNA-hH1neoG2HnRNPA2/B1的成功构建,为进一步研究其基因功能奠定基础。
OBJECTIVE: To construct recombinant HnRNPA2 / B1 plasmid by RNA interference (RNAi) technique and analyze the sequence by parallel analysis. Methods: Reverse transcript sequence of 21bP HnRNPA2 / B1 target sequence and 7bP sequence interval were inserted into PsiRNA-hH1neoG2 plasmid respectively to generate recombinant plasmid PSiRNA-hH1neoG2HnRNPA2 / B1. Results: The recombinant plasmid was sequenced and identified as the desired sequence by sequencing. Conclusion: The successful construction of eukaryotic expression vector PSiRNA-hH1neoG2HnRNPA2 / B1 with short hairpin RNA (shRNA) laid the foundation for further study of its gene function.