论文部分内容阅读
目的研究抗性淀粉(RS)对结(直)肠癌大鼠的肠道功能及变性隐窝病灶(ACF)的影响。方法将50只雄性Wistar大鼠随机分为阴性、阳性对照组及RS低、中、高剂量组。阳性对照组大鼠从实验第2周开始腹腔注射氧化偶氮甲烷(AOM),每周1次,连续2周。阴性对照组注射生理盐水。RS各剂量组致癌剂处理同阳性对照组,并分别自由摄食含7.6%、15.2%及22.8%RS的饲料。阴性和阳性对照组大鼠摄食普通饲料。实验期间每周称重1次,于第5周末和第7周末分别进行粪便pH及排便实验。第16周断头处死所有大鼠,取出结肠组织进行指标ACF的测定。结果与阳性对照组相比,RS各剂量组ACF数目、肠道转运时间(GTT)均明显减少(P<0.01),粪便pH也有所下降(P<0.05),差异有统计学意义。结论RS有利于肠道内的环境健康,降低ACF的发生,可能是预防结(直)肠癌发生的机制之一。
Objective To investigate the effect of resistant starch (RS) on intestinal function and degenerative crypt (ACF) in rats with node (colorectal) cancer. Methods 50 male Wistar rats were randomly divided into negative, positive control group and RS low, medium and high dose groups. The positive control group rats were injected intraperitoneally with oxymethane (AOM) once a week for 2 weeks from the second week of the experiment. Negative control group injected with saline. RS dose groups of carcinogen treatment with the positive control group, and were fed free 7.6%, 15.2% and 22.8% RS feed. Negative and positive control rats fed normal diet. Weighed once a week during the experiment, and stool pH and defecation tests were performed on the 5th and 7th weekend, respectively. At the 16th week, all the rats were decapitated and the colon tissues were removed for the determination of ACF. Results Compared with the positive control group, the numbers of ACF and the transit time of intestinal transit (GTT) in RS groups were significantly decreased (P <0.01) and the stool pH also decreased (P <0.05), the difference was statistically significant. Conclusions RS is beneficial to the environmental health of the intestine and reduces the occurrence of ACF, which may be one of the mechanisms to prevent the occurrence of colorectal cancer.