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目的采用HPLC法同时测定参苓白术胶囊中5种有效成份的含量。方法采用梯度洗脱法测定制剂中去氢土莫酸、猪苓酸C、茯苓酸、薯蓣皂苷元和尿囊素的含量。结果 5.27~105.40μg·m L-1(r=0.9998)去氢土莫酸、4.30~86.00μg·m L-1(r=0.9995)猪苓酸C、5.25~105.00μg·m L-1(r=0.9997)茯苓酸、4.26~85.20μg·m L-1(r=0.9994)薯蓣皂苷元和6.91~138.20μg·m L-1(r=0.9999)尿囊素与其峰面积呈良好的线性关系;平均加样回收率分别为97.77%、97.27%、98.13%、96.93%、99.37%,RSD分别为1.42%、1.07%、0.96%、1.10%、0.56%(n=6)。结论所用方法简便、快捷、重复性好,可用于该制剂的质量控制。
Objective To determine the contents of five active ingredients in Shenling Baizhu Capsule by HPLC simultaneously. Methods The content of deshydroartemolinic acid, albosolic acid C, pachyrin acid, diosgenin and allantoin were determined by gradient elution. Results 5.27 ~ 105.40μg · m L -1 (r = 0.9998), 4.30 ~ 86.00μg · m L -1 (r = 0.9995), and 5.25-105.00 μg · m L -1 r = 0.9997), albinosin with 4.26-85.20 μg · mL -1 (r = 0.9994) and 6.91 ~ 138.20 μg · mL -1 (r = 0.9999) showed a good linear relationship with the peak area The average recoveries were 97.77%, 97.27%, 98.13%, 96.93% and 99.37%, respectively. The RSDs were 1.42%, 1.07%, 0.96%, 1.10% and 0.56%, respectively. Conclusion The method is simple, rapid, reproducible and can be used for the quality control of the preparation.