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目的:建立清肺抑火丸中3个苷类成分的含量标准。方法:采用HPLC,同时以2个检测波长对清肺抑火丸中的黄芩苷、京尼平苷、京尼平龙胆二糖苷进行定量分析。Agilent Zorbax SB-C18色谱柱(4.6 mm×250 mm,5μm);流动相甲醇(A)-1%甲酸溶液(B)-乙腈(C)梯度洗脱,检测波长238,278 nm。结果:黄芩苷、京尼平苷、京尼平龙胆二糖苷分别在0.165～1.650,0.106～1.060,0.035～0.350μg(r>0.999 7)线性关系良好,平均回收率>96.75%,RSD<2.05%。结论:该方法同时进行黄芩苷、京尼平苷、京尼平龙胆二糖苷的定量分析,可作为清肺抑火丸的质量控制标准。
Objective: To establish the content standard of 3 glycosides in Qingfei Yianghu Pills. Methods: Quantitative analysis of baicalin, geniposide and genipin gentiobioside in Qingfei Youtuo Wan was carried out by HPLC with two detection wavelengths. Agilent Zorbax SB-C18 column (4.6 mm × 250 mm, 5 μm); mobile phase of methanol (A) -1% formic acid (B) -acetonitrile (C) gradient elution at 238,278 nm. Results: The linear relationship between baicalin, geniposide and genipin gentiobioside was good at 0.165 ~ 1.650,0.106 ~ 1.060,0.035 ~ 0.350μg (r> 0.999 7), the average recovery was> 96.75%, RSD < 2.05%. Conclusion: The method of simultaneous quantitative analysis of baicalin, geniposide and genipin gentiobioside can be used as the quality control standard of Qingfeiyuhuo pills.