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目的 了解收治SARS病人的定点医院污水中是否含有病毒和病毒核酸。方法 采用载阳电荷滤材吸附浓集污水中的SARS冠状病毒 ,消毒前浓集污水 2 5L ,消毒后 2 5～ 5 0L ;采用VERO细胞培养及RT -PCR技术检测SARS冠状病毒。结果 建立的阳电荷浓集方法对污水中加标f2 噬菌体回收率平均 12 7% ,而SARS冠状病毒回收率只有 1 0 2 %。消毒前在 30 9医院和小汤山医院污水的6d监测中均检测出SARS冠状病毒核酸 ;消毒后 ,在 30 9医院只有 3d监测出病毒核酸 ,其余样品均未检出病毒核酸 ;两医院所有的污水回收液中均未检出活病毒或具有感染性的病毒。结论收治SARS病人医院污水中含有SARS冠状病毒核酸 ,但没有检出活病毒或具有感染性的病毒 ,因此 ,在当时条件下 ,医院污水 ,尤其是消毒后污水传播SARS冠状病毒可能性较小。
Objective To understand whether fixed-point hospital sewage containing SARS patients contain virus and viral nucleic acid. Methods SARS coronavirus was collected from the wastewater by using positive charge filter media. 25 L of sewage was collected before disinfection and 25 to 50 L after disinfection. VERO cell culture and RT-PCR were used to detect SARS coronavirus. Results The positive charge concentration method established an average of 12.7% recovery of spiked bacteriophage in wastewater while the recovery rate of SARS coronavirus was only 102%. SARS coronavirus nucleic acid was detected in 6 days of 30 9 hospital and Xiaotangshan hospital sewage before disinfection. Only 3 days after disinfection, the virus nucleic acid was detected only in 3d hospital and no nucleic acid was detected in other samples. No recoverable live virus or infectious virus was found in the wastewater reclaimed solution. Conclusions SARS coronavirus is contained in the hospital sewage of SARS patients but no live virus or infectious virus is detected. Therefore, under the conditions of the time, SARS coronavirus is unlikely to be transmitted by hospital sewage, especially after disinfection.