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[目的]探讨广西壮族人群谷胱甘肽硫转移酶(glutathione S-transferase,GST)中的GSTM1和GSTT1基因多态性与肺癌易感性的关系。[方法]以病例对照研究方法,采用聚合酶链式反应(PCR)分别检侧58例肺癌患者和60例健康对照的GSTM1、GSTT1基因多态性;χ2检验分析各种基因型频率在肺癌组和对照组之间的差异;用Logistic回归分析吸烟与GSTM1、GSTT1基因型多态性的联合作用。[结果]单独分析GSTM1、GSTT1基因多态性与肺癌相关性无统计学意义,而两者联合则与肺癌有相关性(χ2=4.085,P=0.043)。吸烟与GSTM1缺陷型基因对肺癌易感有协同作用,OR为3.778(95%CI:1.170~12.194,P=0.026);吸烟与GSTT1缺陷型基因对肺癌易感无协同作用,OR为2.833(95%CI:0.982~8.173)。[结论]GSTM1、GSTT1的单一基因多态性不增加患肺癌的危险,而两者联合作用时可增加患肺癌的风险。GSTM1缺陷型有吸烟行为的人更易患肺癌。
[Objective] To investigate the relationship between polymorphisms of GSTM1 and GSTT1 in glutathione S-transferase (GST) and lung cancer susceptibility in Guangxi Zhuang nationality. [Methods] The GSTM1 and GSTT1 gene polymorphisms of 58 lung cancer patients and 60 healthy controls were detected by polymerase chain reaction (PCR) in case-control study. The frequency of genotypes in the lung cancer group And control group. Logistic regression was used to analyze the combined effect of smoking and GSTM1 and GSTT1 genotypes. [Results] There was no significant correlation between GSTM1 and GSTT1 gene polymorphisms and lung cancer when analyzed separately, but the association between them was associated with lung cancer (χ2 = 4.085, P = 0.043). Smoking and GSTM1-deficient genes have a synergistic effect on susceptibility to lung cancer with an OR of 3.778 (95% CI: 1.170-12.124, P = 0.026). Smoking and GSTT1-deficient genes have no synergistic effect on susceptibility to lung cancer with OR of 2.833 % CI: 0.982 ~ 8.173). [Conclusion] The single gene polymorphism of GSTM1 and GSTT1 does not increase the risk of lung cancer, and the combination of the two can increase the risk of lung cancer. People with GSTM1-deficient smokers are more susceptible to lung cancer.