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目的：探讨人大肠癌细胞碱性成纤维细胞生长因子（ｂＦＧＦ）的表达、定位，并检查其受体。方法：采用免疫细胞化学染色、１２５Ｉ标记抗体的原位放射免疫分析及放射受体分析方法。结果：ＲＰＭＩ４７８８细胞产生的ｂＦＧＦ大部分蓄积于细胞核内，一部分存在于细胞膜上，并在细胞膜有其受体。结论：在肿瘤的发生发展过程中，肿瘤细胞产生的ｂＦＧＦ有可能起自泌性生长因子的作用，也可能直接参与核的功能。
Objective: To investigate the expression and localization of basic fibroblast growth factor (bFGF) in human colon cancer cells and examine its receptors. METHODS: Immunocytochemical staining, in situ radioimmunoassay of 125I-labeled antibodies and radioreceptor assays were used. RESULTS: Most of the bFGF produced by RPMI4788 cells accumulated in the nucleus, some of which were present on the cell membrane and had receptors on the cell membrane. Conclusion: During the development of tumors, bFGF produced by tumor cells may play a role in secretory growth factors and may directly participate in the nuclear function.