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体内含巯基(—SH)非蛋白质化台物的主要成分——谷胱甘肽,可通过含硒谷胱甘肽过氧化物酶(Se-GSH-Px)的作用消除氧自由基及其过氧化物。而含巯基蛋白质在维持膜Na~+、K~+-ATP酶正常运转及红细胞膜正常形态等方面具有重要作用。为此,作者采用电流滴定的方法检测了青海黄南地区健康人群血中—SH含量,以探讨衰老与血中巯基含量间的关系。 1 对象与方法 1.1 对象 检测对象为海晏县当地干部、工人与学生,检测前均询问病史、体检,并进行血便常规、X线等项检查,以排除心肺肝肾器官疾患,共计245例(男147例,女98例),年龄16～85岁。 1.2 试剂 测试前,取0.08M硝酸铵水溶液50ml
Glutathione (GSH), the main component of sulfhydryl-free (SH) non-proteome in the body, can eliminate oxygen free radicals and their effects through the action of selenium-containing glutathione peroxidase (Se-GSH-Px) Oxide. The mercapto-containing protein plays an important role in maintaining the normal operation of membrane Na ~ +, K ~ + -ATPase and the normal morphology of erythrocyte membrane. To this end, the author used the current titration method to detect the blood of healthy people in Huangnan area of Qinghai-SH content, in order to explore the relationship between aging and blood thiol content. 1 Subjects and Methods 1.1 Subjects The detection targets were local cadres, workers and students in Haiyan County. Before the test, patients were asked about their medical history and physical examination. Blood routine examination and X-ray examination were performed to exclude cardiopulmonary, 147 males and 98 females) aged 16-85 years old. 1.2 reagent test, take 0.08M ammonium nitrate solution 50ml