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目的建立垂盆草片的质量标准。方法采用薄层色谱法对处方中垂盆草进行定性鉴别,采用高效液相色谱法同时测定制剂中的槲皮素、山柰素、异鼠李素的含量。结果薄层色谱斑点清晰,阴性对照无干扰。测定槲皮素、山柰素、异鼠李素含量分别在0.032~0.640μg/mL、0.011~0.220μg/mL和0.008~0.160μg/mL范围内有良好的线性关系,相关系数分别为1.000 0,0.999 9,0.999 9,平均回收率分别为97.47%(RSD为1.67%)、96.98%(RSD为1.64%)和97.69%(RSD为2.37%)。结论所建立的方法可靠,能准确地进行定性、定量检测,可用于该制剂的质量控制。
Objective To establish the quality standard of Prismatic Tablets. Methods Thin layer chromatography (HPLC) was used to qualitatively identify stilbe concanavalin in the prescription. The contents of quercetin, kaempferol and isorhamnetin in the preparations were determined simultaneously by high performance liquid chromatography. Results TLC spots clear, negative control without interference. The determination of quercetin, kaempferol, isorhamnetin were 0.032 ~ 0.640μg / mL, 0.011 ~ 0.220μg / mL and 0.008 ~ 0.160μg / mL within a good linear relationship, the correlation coefficients were 1.000 0 , 0.999 9,0.9999 respectively. The average recoveries were 97.47% (RSD 1.67%), 96.98% (RSD 1.64%) and 97.69% (RSD 2.37%), respectively. Conclusion The established method is reliable, can be accurately qualitative and quantitative detection, can be used for the quality control of the preparation.