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应用ABC免疫组化及半巢式PCR分别对大肠癌bc1-2基因表达及重排进行了检测。结果表明 ,bc1-2基因异常表达与重排在大肠癌早期即已出现明显的异常改变。随着病程演变 ,这种异常现象表现增多趋势即愈晚期重排发生率显著增加。而bc1-2基因的蛋白在有淋巴结转移组亦明显多于无淋巴结转移组 ,说明bc1-2基因参与大肠癌发生发展过程的调节 ,并在大肠癌细胞的增殖、进展及转移中起重要作用。研究大肠癌bc1-2基因情况可用来预测治疗效果和预后 ,亦可为大肠癌治疗设计新的抗癌药物提供了理论依据。两法联合应用相比较 ,以重排更能反映大肠癌分子特性
ABC immunohistochemistry and semi-nested PCR were used to detect the expression and rearrangement of bc1-2 gene in colorectal cancer. The results showed that abnormal expression and rearrangement of the bcl-2 gene in the early stages of colorectal cancer have already shown significant abnormal changes. As the course of the disease evolves, this abnormal phenomenon manifests itself in an increasing tendency that the rate of late rearrangement increases significantly. The bcl-2 gene protein was also significantly more in the group with lymph node metastasis than in the group without lymph node metastasis, indicating that the bcl-2 gene is involved in the regulation of the development of colorectal carcinoma and plays an important role in the proliferation, progression and metastasis of colorectal cancer cells. . To study the status of colorectal cancer bcl-2 gene can be used to predict the treatment effect and prognosis, and also provide a theoretical basis for the design of new anticancer drugs for the treatment of colorectal cancer. Comparison of the two methods combined to rearrange the molecular characteristics of colorectal cancer